Two nuclear localization signals regulate intracellular localization of the duck enteritis virus UL13 protein

Duck enteritis virus (DEV) multifunctional tegument protein UL13 is predicted to be a conserved herpesvirus kinase; however, little known about its subcellular localization signal. In this study, through transfection of 2 nuclear signals DEV fused enhanced green fluorescent protein, bipartite (NLS) were identified. We found that ivermectin blocked the NLS-mediated import UL13, showing signal classical importin α- and β-dependent process. constructed mutant strain in which NLS was deleted explore whether deletion affects viral replication. Amino acids 4 7 90 96 NLSs, further proving occurs via also pUL13 had no effect on replication cell culture. Our study enhances understanding pUL13. Taken together, these results provide significant information regarding biological function during infection.